Who BSL-2 requirements?

BSL–2

  • Appropriate personal protective equipment (PPE) must be worn, including lab coats and gloves.
  • All procedures that can cause infection from aerosols or splashes are performed within a biological safety cabinet (BSC).
  • An autoclave or an alternative method of decontamination is available for proper disposals.

What are BSL-2 pathogens?

Examples of BSL-2 organisms are: Mycobacterium, Streptococcus pneumonia, Salmonella choleraesuis. BSL-3 risk group contains biological agents that usually cause serious disease (human, animal or plant) or that can result in serious economic consequences. These agents are usually not spread by casual contact.

What does it mean to be ranked BSL-2?

Lab Space. Biosafety levels BSL-1 through BSL-4 are ranked from the lowest potential hazard (BSL-1), or basic laboratories, to the highest potential hazard (BSL-4), with maximum containment. BSL-2 laboratories have containment and BSL-3 have high containment.

Is BSL-2 a blood?

BSL-2 is the biosafety level used for work with human blood, body fluids, or tissues where the presence of an infectious agent may be unknown. Primary hazards at BSL-2 include accidental percutaneous or mucous membrane exposures, exposure of non-intact skin, or ingestion of infectious materials.

Which of the following material’s must be handled at BSL-2?

BSL-2 precautions are suitable for work involving agents that pose moderate hazards to personnel and the environment. They are appropriate when work is done with any human-derived blood, body fluids, tissues, or primary human cell lines where the presence of an infectious agent may be unknown.

Is recombinant DNA BSL-2?

Recombinant DNA activity requiring BSL-2 physical containment including animal studies that involve the construction of transgenic animals. Non-recombinant cell and/or tissue culture systems that require this level of containment. Oncogenic viral systems classified as low risk.

Which facilities are required for working in a BSL-2 laboratory?

In a BSL-2 lab, the following conditions are to exist:

  • Doors. Doors that can be locked and secured should be installed for facilities that house restricted areas.
  • Public.
  • Sink.
  • Cleaning.
  • Bench Tops.
  • Lab Furniture.
  • Biological Safety Cabinets.
  • Eyewash Station.