What is PCR SSOP?

The combination of PCR technology and hybridization with sequence-specific oligonucleotide probes was first applied to HLA class II typing because of the limitations of DR serology and of the better knowledge of allelic polymorphism at DR/DQ loci. …

What is SSO typing?

Abstract. Human leukocyte antigen (HLA) typing, utilising the sequence-specific oligonucleotide (SSO) and sequence-specific primer (SSP) technologies, has been in routine use in many tissue typing laboratories worldwide for more than 20 years since the development of the polymerase chain reaction.

What is sequence-specific oligonucleotide?

The sequence-specific oligonucleotide (SSO) probe is capable of detecting single nucleotide mismatch with a high degree of efficiency that has been in use for some time. Their combination has been well documented in the analysis of unamplified genomic DNA.

What is sequence specific oligonucleotide probe?

are used in many important hybridization techniques including DNA microarrays designed for large-scale variation analysis and genotyping of complex DNA samples.

How do oligonucleotide probes work?

Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. Oligonucleotide probes are usually labelled, for example with radioisotopes, epitopes, biotin or fluorophores to enable their detection.

What does a positive HLA test mean?

A positive test means HLA-B27 is present. It suggests a greater-than-average risk for developing or having certain autoimmune disorders. An autoimmune disorder is a condition that occurs when the immune system mistakenly attacks and destroys healthy body tissue.

How do oligonucleotides work in PCR?

Oligonucleotides made up of 2′-deoxyribonucleotides are the molecules used in polymerase chain reaction (PCR). These are referred to as primers and are used to massively amplify a small amount of DNA.