What is BSA from SDS-PAGE?

Bovine Serum Albumin (BSA) has been used: as a standard protein in the elution-filter aided proteome preparation (FASP) method and in sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) as a component of the molecular weight marker in SDS-PAGE and western blotting.

Why is BSA used in SDS-PAGE?

Sodium dodecyl sulfate (SDS) and bovine serum albumin (BSA) are the most studied system. This surfactant binds strongly to the macromolecule, inducing its partial unfolding. A practical use of SDS/BSA is in the polyacrylamide gel electrophoresis methodology for determining molecular weights of proteins.

Does SDS-PAGE give molecular weight?

Based on the values obtained for the bands in the standard, the logarithm of the molecular weight of an SDS-denatured polypeptide and its relative migration distance (Rf) is plotted into a graph. Interpolating the value from this graph will then give you the molecular weight of the unknown protein band.

What is BSA in electrophoresis?

The relative migrations of protein bands in the standards were used to estimate the molecular weight of bovine serum albumin (BSA) on western blots generated from each gel type.

What is the molecular weight of BSA?

The molecular weight of BSA has frequently been cited as 66,1201 or 66,2672, but it was revised in 1990 to 66,4303. All three values are based on amino acid sequence information available at the time of publication.

Where is BSA found?

cow
BSA is a protein found predominantly in the circulatory system of the cow but is also a constituent of the whey component of bovine milk.

What is the molecular weight range of protein?

The broad range protein molecular standards include 11 proteins from 6500 to 205,000 daltons for use as molecular weight standards in SDS-polyacrylamide gel electrophoresis.

How does SDS-PAGE calculate protein concentration?

You can use densitometer to find and compare the denisty (which is related to concentration) of protein together. Also, you can scan the SDS-PAGE with high resulotion scanner and then compare band density and volume with some software such as Melanie.

How many KD is BSA?

66.5 kDa
Physical properties of BSA: Number of amino acid residues: 583. Molecular weight: 66,463 Da (= 66.5 kDa)

What is BSA in molecular biology?

Bovine Serum Albumin (BSA) is supplied with some products to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation.

What is BSA used for?

BSA (often from a fetal bovine source) is also used as a nutrient in cell and microbial culture. In molecular biology, BSA is used to stabilize some restriction enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels.

Does BSA expire?

THIS PRODUCT IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE. To effectively preserve the integrity of BSA, it should be stored at the recommended temperature and protected from light. For stability and optimal performance, this product should be stored at 2 to 8 C. Do not use after the expiration date.